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KMID : 0368420070500020156
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Journal of Plant Biology
2007 Volume.50 No. 2 p.156 ~ p.160
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Anthocyanin content in rice is related to expression levels of anthocyanin biosynthetic genes
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Kim Bong-Gyu
Kim Jeong-Ho
Min Shin-Young
Shin Kwang-Hee
Kim Ji-Hye
Kim Hong-Yeol
Ryu Su-Noh
Ahn Joong-Hoon
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Abstract
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The composition of anthocyanins was analyzed in two rice cultivais, ‘llpum’ showed no detectable levels, while ‘Heugjinju’ contained three types of anthocyanins: cyanidin, cyanidin 3-O-glucoside, and peonidin 3-O-glucoside. We also assessed the expression of anthocyanin biosynthetic genes ? for phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), flavanone 3β-hydroxylase (F3H), dihydroflavonol reductase (DFR), and anthocyanin synthase (ANS)-in different tissues of those cultivars. All five genes were expressed more in the leaves and seeds of ‘Heugjinju’ than in ‘Ilpum’, with the greatest amount of transcript being detected in ‘Heugjinju’ seeds. Two genes,DFR andANS, had relatively high expression levels and were specific for anthocyanin biosynthesis. Furthermore, expression of CHS,F3H, DFR, andANS was enhanced during seed maturation and was correlated with ambient temperature during seedling growth.
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KEYWORD
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anthocyanin biosynthesis, Oryza sativa, real-time PCR
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